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Assay Detail

CHEMBL5353415

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ZIKV NS5 MTase activity assessed as methyltransferase reaction using capped RNA substrate pre-incubated for 2.5 hrs followed by enzyme addition measured after 1 hrs by luminometer
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Zika virus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Genome polyprotein (CHEMBL4523307)
Type SINGLE PROTEIN
Organism Zika virus

Publication

Discovery of dehydroandrographolide derivatives with C19 hindered ether as potent anti-ZIKV agents with inhibitory activities to MTase of ZIKV NS5.
Qian W, Zhou GF, Ge X, Xue JX, Zheng CB, Yang LM, Gu XR, Li C, Xiong SD, Zheng YT, Zhou GC.
Eur J Med Chem (2022) 243 : 114710 -114710
PubMed 36055002 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.68 4.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5421176 1 4.85
CHEMBL5402544 1 4.74
CHEMBL1214186 SINEFUNGIN 2.0 1 4.72
CHEMBL4206100 1 4.41
CHEMBL5414044 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5421176 IC50 = 14130.0 nM 4.85
CHEMBL5402544 IC50 = 18340.0 nM 4.74
CHEMBL1214186 SINEFUNGIN IC50 = 19200.0 nM 4.72
CHEMBL4206100 IC50 = 38860.0 nM 4.41
CHEMBL5414044 IC50 = 183250.0 nM -