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Assay Detail

CHEMBL5356858

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Partial agonist activity against mouse MC1R expressed in HEK293 cells assessed as increase in cAMP level incubated for 2 hrs by AlphaScreen assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocyte-stimulating hormone receptor (CHEMBL4077)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of a Pan-Melanocortin Receptor Antagonist [Ac-DPhe(pI)-Arg-Nal(2')-Orn-NH<sub>2</sub>] at the MC1R, MC3R, MC4R, and MC5R that Mediates an Increased Feeding Response in Mice and a 40-Fold Selective MC1R Antagonist [Ac-DPhe(pI)-DArg-Nal(2')-Arg-NH<sub>2</sub>].
Ericson MD, Tran LT, Mathre SS, Freeman KT, Holdaway K, John K, Lunzer MM, Bouchard JL, Haskell-Luevano C.
J Med Chem (2023) 66 : 8103 -8117
PubMed 37307241 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 5.96 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5417913 1 6.40
CHEMBL5403689 1 5.96
CHEMBL5421386 1 5.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5417913 EC50 = 400.0 nM 6.40
CHEMBL5403689 EC50 = 1100.0 nM 5.96
CHEMBL5421386 EC50 = 3000.0 nM 5.52