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Assay Detail

CHEMBL5356873

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at mouse MC3R expressed in HEK293 cells assessed as reduction in NDP-MSH induced cAMP formation incubated for 2 hrs by AlphaScreen based schild plot analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 3 (CHEMBL4774)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of a Pan-Melanocortin Receptor Antagonist [Ac-DPhe(pI)-Arg-Nal(2')-Orn-NH<sub>2</sub>] at the MC1R, MC3R, MC4R, and MC5R that Mediates an Increased Feeding Response in Mice and a 40-Fold Selective MC1R Antagonist [Ac-DPhe(pI)-DArg-Nal(2')-Arg-NH<sub>2</sub>].
Ericson MD, Tran LT, Mathre SS, Freeman KT, Holdaway K, John K, Lunzer MM, Bouchard JL, Haskell-Luevano C.
J Med Chem (2023) 66 : 8103 -8117
PubMed 37307241 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 22 7.08 9.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3422426 1 9.20
CHEMBL5403689 1 7.80
CHEMBL5405613 1 7.70
CHEMBL4865888 1 7.50
CHEMBL5408661 1 7.40
CHEMBL5421386 1 7.40
CHEMBL5419394 1 7.40
CHEMBL5413452 1 7.30
CHEMBL5417913 1 7.00
CHEMBL5413535 1 6.80
CHEMBL5414015 1 6.70
CHEMBL5397924 1 6.60
CHEMBL5406385 1 6.50
CHEMBL4866219 1 6.50
CHEMBL5409282 1 5.80
CHEMBL5397668 1 5.70
CHEMBL5396157 1 -
CHEMBL5413381 1 -
CHEMBL5435335 1 -
CHEMBL5397260 1 -
CHEMBL5421355 1 -
CHEMBL5438129 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3422426 Kd = 0.631 nM 9.20
CHEMBL5403689 Kd = 15.85 nM 7.80
CHEMBL5405613 Kd = 19.95 nM 7.70
CHEMBL4865888 Kd = 31.62 nM 7.50
CHEMBL5408661 Kd = 39.81 nM 7.40
CHEMBL5421386 Kd = 39.81 nM 7.40
CHEMBL5419394 Kd = 39.81 nM 7.40
CHEMBL5413452 Kd = 50.12 nM 7.30
CHEMBL5417913 Kd = 100.0 nM 7.00
CHEMBL5413535 Kd = 158.49 nM 6.80
CHEMBL5414015 Kd = 199.53 nM 6.70
CHEMBL5397924 Kd = 251.19 nM 6.60
CHEMBL5406385 Kd = 316.23 nM 6.50
CHEMBL4866219 Kd = 316.23 nM 6.50
CHEMBL5409282 Kd = 1584.89 nM 5.80
CHEMBL5397668 Kd = 1995.26 nM 5.70
CHEMBL5396157 Kd > 3162.28 nM -
CHEMBL5413381 Kd > 3162.28 nM -
CHEMBL5435335 Kd > 3162.28 nM -
CHEMBL5397260 Kd > 3162.28 nM -
CHEMBL5421355 Kd > 3162.28 nM -
CHEMBL5438129 Kd > 3162.28 nM -