Assay Detail
Binding
CHEMBL5357335
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human DHFR using DHF as substrate assessed as inhibition constant in presence of NADPH by UV-Vis spectrometry analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of rigid biphenyl <i>Plasmodium falciparum</i> DHFR inhibitors using a fragment linking strategy.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 9 | 7.18 | 7.78 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5421199 | — | — | 1 | 7.78 |
| CHEMBL3040038 | — | — | 1 | 7.76 |
| CHEMBL5415214 | — | — | 1 | 7.71 |
| CHEMBL5432011 | — | — | 1 | 7.66 |
| CHEMBL5397904 | — | — | 1 | 6.96 |
| CHEMBL5406769 | — | — | 1 | 6.86 |
| CHEMBL5417784 | — | — | 1 | 6.83 |
| CHEMBL5404134 | — | — | 1 | 6.67 |
| CHEMBL5440218 | — | — | 1 | 6.37 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5421199 | — | Ki | = | 16.58 | nM | 7.78 |
| CHEMBL3040038 | — | Ki | = | 17.41 | nM | 7.76 |
| CHEMBL5415214 | — | Ki | = | 19.4 | nM | 7.71 |
| CHEMBL5432011 | — | Ki | = | 22.0 | nM | 7.66 |
| CHEMBL5397904 | — | Ki | = | 108.4 | nM | 6.96 |
| CHEMBL5406769 | — | Ki | = | 139.78 | nM | 6.86 |
| CHEMBL5417784 | — | Ki | = | 147.13 | nM | 6.83 |
| CHEMBL5404134 | — | Ki | = | 214.41 | nM | 6.67 |
| CHEMBL5440218 | — | Ki | = | 422.92 | nM | 6.37 |