Assay Detail
Functional
CHEMBL5358336
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Agonist activity at NPFF2R (unknown origin) transfected in HEK293 cells co-expressing Galphaq15 assessed as increase in calcium mobilization by Fluo-4AM dye based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
All-Hydrocarbon Stapled Peptide Multifunctional Agonists at Opioid and Neuropeptide FF Receptors: Highly Potent, Long-Lasting Brain Permeant Analgesics with Diminished Side Effects.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 8 | 6.33 | 7.48 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5435869 | — | — | 1 | 7.48 |
| CHEMBL5410237 | — | — | 1 | 7.28 |
| CHEMBL429731 | — | — | 1 | 7.10 |
| CHEMBL5428360 | — | — | 1 | 6.84 |
| CHEMBL5401796 | — | — | 1 | 5.97 |
| CHEMBL5430632 | — | — | 1 | 5.51 |
| CHEMBL5427400 | — | — | 1 | 5.42 |
| CHEMBL5406732 | — | — | 1 | 5.01 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5435869 | — | EC50 | = | 33.1 | nM | 7.48 |
| CHEMBL5410237 | — | EC50 | = | 52.3 | nM | 7.28 |
| CHEMBL429731 | — | EC50 | = | 78.9 | nM | 7.10 |
| CHEMBL5428360 | — | EC50 | = | 144.3 | nM | 6.84 |
| CHEMBL5401796 | — | EC50 | = | 1076.0 | nM | 5.97 |
| CHEMBL5430632 | — | EC50 | = | 3110.0 | nM | 5.51 |
| CHEMBL5427400 | — | EC50 | = | 3756.0 | nM | 5.42 |
| CHEMBL5406732 | — | EC50 | = | 9766.0 | nM | 5.01 |