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Assay Detail

CHEMBL5359744

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human ALDH1A1 using propionaldehyde as substrate preincubated with enzyme for 15 mins followed by substrate addition in presence of NAD+ and measured for 5 mins by spectrophotometric analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aldehyde dehydrogenase 1A1 (CHEMBL3577)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Scaffold hopping based designing of selective ALDH1A1 inhibitors to overcome cyclophosphamide resistance: synthesis and biological evaluation.
Narendra G, Raju B, Verma H, Kumar M, Jain SK, Tung GK, Thakur S, Kaur R, Kaur S, Sapra B, Silakari O.
RSC Med Chem (2024) 15 : 309 -321
PubMed 38283216 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.93 6.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5396348 1 6.50
CHEMBL5403165 1 6.26
CHEMBL4215649 1 6.05
CHEMBL1453086 1 5.79
CHEMBL1922082 1 5.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5396348 IC50 = 320.0 nM 6.50
CHEMBL5403165 IC50 = 550.0 nM 6.26
CHEMBL4215649 IC50 = 900.0 nM 6.05
CHEMBL1453086 IC50 = 1630.0 nM 5.79
CHEMBL1922082 IC50 = 8670.0 nM 5.06