Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5360734

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of eNOS in human DLD-1 cells assessed as reduction of L-[14C]arginine to L-[14C]citrulline conversion
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type DLD-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase 3 (CHEMBL4803)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chemical similarities and differences among inhibitors of nitric oxide synthase, arginase and dimethylarginine dimethylaminohydrolase-1: Implications for the design of novel enzyme inhibitors modulating the nitric oxide pathway.
Doman AJ, Tommasi S, Perkins MV, McKinnon RA, Mangoni AA, Nair PC.
Bioorg Med Chem (2022) 72 : 116970 -116970
PubMed 36063653 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 5.91 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL483091 1 7.66
CHEMBL321691 ETHYLISOTHIOUREA 1 7.41
CHEMBL483092 1 6.17
CHEMBL107201 1 5.05
CHEMBL505665 BENZYL CARBAMIMIDOTHIOATE 1 4.64
CHEMBL301607 1 4.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL483091 Ki = 22.0 nM 7.66
CHEMBL321691 ETHYLISOTHIOUREA Ki = 39.0 nM 7.41
CHEMBL483092 Ki = 670.0 nM 6.17
CHEMBL107201 Ki = 9000.0 nM 5.05
CHEMBL505665 BENZYL CARBAMIMIDOTHIOATE Ki = 23000.0 nM 4.64
CHEMBL301607 Ki = 31000.0 nM 4.51