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Assay Detail

CHEMBL5361387

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to recombinant human CD73 expressed in Sf9 cells preincubated for 25 mins and measured after 30 mins by liquid scintillation method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5'-nucleotidase (CHEMBL5957)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Targeting ecto-5'-nucleotidase: A comprehensive review into small molecule inhibitors and expression modulators.
das Neves GM, Kagami LP, Battastini AMO, Figueiró F, Eifler-Lima VL.
Eur J Med Chem (2023) 247 : 115052 -115052
PubMed 36599229 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 6.39 6.73

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5421717 1 6.73
CHEMBL1206141 1 6.35
CHEMBL5437993 1 6.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5421717 Ki = 185.0 nM 6.73
CHEMBL1206141 Ki = 451.0 nM 6.35
CHEMBL5437993 Ki = 831.0 nM 6.08