Assay Detail
Binding
CHEMBL5363647
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of DGK in human whole blood cell assessed as increase in IFNgamma level incubated for 1 hr followed by anti-human CD3/ antiCD28 mAB stimulation and measure after 24 hrs by AlphaLISA assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Tissue | Blood |
| Cell Type | Whole blood cell |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Discovery of Potent, Dual-Inhibitors of Diacylglycerol Kinases Alpha and Zeta Guided by Phenotypic Optimization.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 6.55 | 6.55 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5424446 | — | — | 1 | 6.55 |
| CHEMBL5408449 | — | — | 1 | - |
| CHEMBL261131 | DIACYLGLYCEROL KINASE INHIBITOR II | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5424446 | — | EC50 | = | 280.0 | nM | 6.55 |
| CHEMBL5408449 | — | EC50 | > | 20000.0 | nM | - |
| CHEMBL261131 | DIACYLGLYCEROL KINASE INHIBITOR II | EC50 | > | 10000.0 | nM | - |