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Assay Detail

CHEMBL5363740

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of Cy5-labeled BIM BH3 peptide (H3N-(C/Cy5Mal)-WIAQELRRIGDEFN-OH) from MCL-1 (unknown origin) assessed as inhibition constant incubated for 60 mins by HTRF assay
14
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Macrocyclic Carbon-Linked Pyrazoles As Novel Inhibitors of MCL-1.
Demin S, Peschiulli A, Velter AI, Vos A, De Boeck B, Miller B, Rombouts FJR, Reuillon T, Lento W, Blanco MD, Jouffroy M, Steyvers H, Bekkers M, Altrocchi C, Pietrak B, Koo SJ, Szewczuk L, Attar R, Philippar U.
ACS Med Chem Lett (2023) 14 : 955 -961
PubMed 37465311 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 10.38 10.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5396628 1 10.89
CHEMBL5307201 1 10.82
CHEMBL5400376 1 10.74
CHEMBL5402764 1 10.70
CHEMBL5426737 1 10.70
CHEMBL5437853 1 10.66
CHEMBL5412641 1 10.64
CHEMBL5394656 1 10.49
CHEMBL5437622 1 10.47
CHEMBL5433387 1 10.40
CHEMBL5405517 1 10.39
CHEMBL5402950 1 10.33
CHEMBL5403706 2 10.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5396628 Ki = 0.013 nM 10.89
CHEMBL5307201 Ki = 0.015 nM 10.82
CHEMBL5400376 Ki = 0.018 nM 10.74
CHEMBL5402764 Ki = 0.02 nM 10.70
CHEMBL5426737 Ki = 0.02 nM 10.70
CHEMBL5437853 Ki = 0.022 nM 10.66
CHEMBL5412641 Ki = 0.023 nM 10.64
CHEMBL5394656 Ki = 0.032 nM 10.49
CHEMBL5437622 Ki = 0.034 nM 10.47
CHEMBL5433387 Ki = 0.04 nM 10.40
CHEMBL5405517 Ki = 0.041 nM 10.39
CHEMBL5402950 Ki = 0.047 nM 10.33
CHEMBL5403706 Ki = 0.069 nM 10.16
CHEMBL5403706 Ki = 11.0 nM 7.96