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Assay Detail

CHEMBL5363883

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at SNAP-tagged human D3R receptor transfected in HEK293T cells coexpressing wild type Galpha-oA, venus-tagged Gbeta-1 (156 to 239 residues), venus-tagged Ggamma2 (1 to 155 residues) and Rluc8 fused masGRK3ct assessed as Galpha-oA activation using coelenterazine as substrate incubated for 40 mins in presence of quinpirole by BRET based GPA assay
25
Total Activities
13
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(3) dopamine receptor (CHEMBL234)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pharmacological and Physicochemical Properties Optimization for Dual-Target Dopamine D<sub>3</sub> (D<sub>3</sub>R) and μ-Opioid (MOR) Receptor Ligands as Potentially Safer Analgesics.
Bonifazi A, Saab E, Sanchez J, Nazarova AL, Zaidi SA, Jahan K, Katritch V, Canals M, Lane JR, Newman AH.
J Med Chem (2023) 66 : 10304 -10341
PubMed 37467430 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.48 8.56
EC50 12 6.48 8.56
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL54 HALOPERIDOL 4.0 2 8.56
CHEMBL5414796 2 7.10
CHEMBL5395539 2 7.04
CHEMBL5399270 2 6.57
CHEMBL5433458 2 6.57
CHEMBL5412606 2 6.50
CHEMBL5404724 2 6.41
CHEMBL5415842 2 6.25
CHEMBL5412995 2 6.21
CHEMBL5397609 2 5.76
CHEMBL5434650 2 5.53
CHEMBL5398913 2 5.26
CHEMBL5432993 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL54 HALOPERIDOL IC50 = 2.754 nM 8.56
CHEMBL54 HALOPERIDOL EC50 = 2.76 nM 8.56
CHEMBL5414796 IC50 = 79.43 nM 7.10
CHEMBL5414796 EC50 = 78.9 nM 7.10
CHEMBL5395539 EC50 = 90.9 nM 7.04
CHEMBL5395539 IC50 = 91.2 nM 7.04
CHEMBL5433458 EC50 = 271.0 nM 6.57
CHEMBL5433458 IC50 = 269.15 nM 6.57
CHEMBL5399270 IC50 = 269.15 nM 6.57
CHEMBL5399270 EC50 = 269.0 nM 6.57
CHEMBL5412606 IC50 = 316.23 nM 6.50
CHEMBL5412606 EC50 = 316.0 nM 6.50
CHEMBL5404724 EC50 = 386.0 nM 6.41
CHEMBL5404724 IC50 = 389.05 nM 6.41
CHEMBL5415842 IC50 = 562.34 nM 6.25
CHEMBL5415842 EC50 = 566.0 nM 6.25
CHEMBL5412995 IC50 = 616.6 nM 6.21
CHEMBL5412995 EC50 = 616.0 nM 6.21
CHEMBL5397609 IC50 = 1737.8 nM 5.76
CHEMBL5397609 EC50 = 1737.0 nM 5.76
CHEMBL5434650 IC50 = 2951.21 nM 5.53
CHEMBL5434650 EC50 = 2962.0 nM 5.53
CHEMBL5398913 EC50 = 5446.0 nM 5.26
CHEMBL5398913 IC50 = 5495.41 nM 5.26
CHEMBL5432993 Activity - -