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Assay Detail

CHEMBL5364102

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Binding
Binding affinity to N-terminal Avi-tagged and C-terminal His6-tagged USP3 (1 to 131 residues) (unknown origin) expressed in Escherichia coli BirA assessed as dissociation constant by SPR analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ubiquitin carboxyl-terminal hydrolase 3 (CHEMBL5291515)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Characterization of a Chemical Probe Targeting the Zinc-Finger Ubiquitin-Binding Domain of HDAC6.
Harding RJ, Franzoni I, Mann MK, Szewczyk MM, Mirabi B, Ferreira de Freitas R, Owens DDG, Ackloo S, Scheremetjew A, Juarez-Ornelas KA, Sanichar R, Baker RJ, Dank C, Brown PJ, Barsyte-Lovejoy D, Santhakumar V, Schapira M, Lautens M, Arrowsmith CH.
J Med Chem (2023) 66 : 10273 -10288
PubMed 37499118 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 2 4.65 4.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5410606 1 4.96
CHEMBL5426708 1 4.33

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5410606 Kd = 11000.0 nM 4.96
CHEMBL5426708 Kd = 47000.0 nM 4.33