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Assay Detail

CHEMBL5364128

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at N-terminal NanoLuc-tagged USP16 R84A/Y117A mutant (unknown origin)/N-terminal HaloTag-tagged ISGI5 (unknown origin ) transfected in human HEK293T cells assessed as inhibition of protein-protein interaction preincubated for 240 min followed by Nano-Glo substrate addition and measured for 10 mins by NanoBRET assay
2
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery and Characterization of a Chemical Probe Targeting the Zinc-Finger Ubiquitin-Binding Domain of HDAC6.
Harding RJ, Franzoni I, Mann MK, Szewczyk MM, Mirabi B, Ferreira de Freitas R, Owens DDG, Ackloo S, Scheremetjew A, Juarez-Ornelas KA, Sanichar R, Baker RJ, Dank C, Brown PJ, Barsyte-Lovejoy D, Santhakumar V, Schapira M, Lautens M, Arrowsmith CH.
J Med Chem (2023) 66 : 10273 -10288
PubMed 37499118 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 4.70 4.70
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5410606 1 4.70
CHEMBL5426708 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5410606 EC50 = 20000.0 nM 4.70
CHEMBL5426708 Activity - -