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Assay Detail

CHEMBL5365103

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to N-terminal 6His-tagged full length truncated SHMT1 (unknown origin) expressed in Escherichia coli Rosetta (DE3) pLysS assessed as inhibition constant by spectrophotometric analysis
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine hydroxymethyltransferase, cytosolic (CHEMBL1772927)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design of Transport-Specific Multitargeted One-Carbon Metabolism Inhibitors in Cytosol and Mitochondria.
Nayeen MJ, Katinas JM, Magdum T, Shah K, Wong JE, O'Connor CE, Fifer AN, Wallace-Povirk A, Hou Z, Matherly LH, Dann CE, Gangjee A.
J Med Chem (2023) 66 : 11294 -11323
PubMed 37582241 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 5.86 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5438587 1 7.22
CHEMBL5429283 1 6.92
CHEMBL5440815 1 6.66
CHEMBL5408906 1 6.10
CHEMBL5431881 1 6.05
CHEMBL5417651 1 5.98
CHEMBL5402700 1 5.98
CHEMBL5427804 1 5.96
CHEMBL5405010 1 5.90
CHEMBL5431331 1 5.82
CHEMBL5421259 1 5.51
CHEMBL5415985 1 5.15
CHEMBL5418034 1 5.04
CHEMBL5432700 1 4.88
CHEMBL5400671 1 4.74
CHEMBL34259 METHOTREXATE 4.0 1 -
CHEMBL1201746 PRALATREXATE 4.0 1 -
CHEMBL225072 PEMETREXED 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5438587 Ki = 60.0 nM 7.22
CHEMBL5429283 Ki = 120.0 nM 6.92
CHEMBL5440815 Ki = 220.0 nM 6.66
CHEMBL5408906 Ki = 790.0 nM 6.10
CHEMBL5431881 Ki = 900.0 nM 6.05
CHEMBL5417651 Ki = 1040.0 nM 5.98
CHEMBL5402700 Ki = 1040.0 nM 5.98
CHEMBL5427804 Ki = 1110.0 nM 5.96
CHEMBL5405010 Ki = 1250.0 nM 5.90
CHEMBL5431331 Ki = 1530.0 nM 5.82
CHEMBL5421259 Ki = 3120.0 nM 5.51
CHEMBL5415985 Ki = 7100.0 nM 5.15
CHEMBL5418034 Ki = 9190.0 nM 5.04
CHEMBL5432700 Ki = 13270.0 nM 4.88
CHEMBL5400671 Ki = 18210.0 nM 4.74
CHEMBL34259 METHOTREXATE Ki > 200000.0 nM -
CHEMBL1201746 PRALATREXATE Ki > 200000.0 nM -
CHEMBL225072 PEMETREXED Ki > 200000.0 nM -