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Assay Detail

CHEMBL5366015

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to NanoLuc fused Snap CXCR2 (unknown origin) expressed in HEK293 cell membrane assessed as inhibition constant using furimazine as substrate incubated with substrate for 5 mins and measured every 15 secs for 60 mins by luminescence based NanoBRET fluorescent binding assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-X-C chemokine receptor type 2 (CHEMBL2434)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Application of Fluorescent Ligands Targeting the Intracellular Allosteric Binding Site of the CXC Chemokine Receptor 2.
Casella BM, Farmer JP, Nesheva DN, Williams HEL, Charlton SJ, Holliday ND, Laughton CA, Mistry SN.
J Med Chem (2023) 66 : 12911 -12930
PubMed 37523859 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 8.50 9.61

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4562140 1 9.61
CHEMBL216981 NAVARIXIN ANHYDROUS 2.0 1 9.19
CHEMBL5421394 1 8.87
CHEMBL3039531 DANIRIXIN 2.0 1 8.71
CHEMBL38182 1 8.68
CHEMBL218964 1 8.48
CHEMBL403225 1 8.32
CHEMBL5424054 1 8.31
CHEMBL5404638 1 8.16
CHEMBL1162935 1 8.06
CHEMBL5398802 1 7.97
CHEMBL5423846 1 7.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4562140 Ki = 0.2455 nM 9.61
CHEMBL216981 NAVARIXIN ANHYDROUS Ki = 0.6457 nM 9.19
CHEMBL5421394 Ki = 1.349 nM 8.87
CHEMBL3039531 DANIRIXIN Ki = 1.95 nM 8.71
CHEMBL38182 Ki = 2.089 nM 8.68
CHEMBL218964 Ki = 3.311 nM 8.48
CHEMBL403225 Ki = 4.786 nM 8.32
CHEMBL5424054 Ki = 4.898 nM 8.31
CHEMBL5404638 Ki = 6.918 nM 8.16
CHEMBL1162935 Ki = 8.71 nM 8.06
CHEMBL5398802 Ki = 10.72 nM 7.97
CHEMBL5423846 Ki = 23.99 nM 7.62