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Assay Detail

CHEMBL5366245

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SphK1 (unknown origin) using D-Sph as substrate incubated for 40 mins by ADP-Glo Plus luminescence kinase assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sphingosine kinase 1 (CHEMBL4394)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, antitumor activity, and molecular dynamics simulations of novel sphingosine kinase 2 inhibitors.
Wang S, Huo Y, Zhang J, Li L, Cao F, Song Y, Zhang Y, Yang K.
Bioorg Med Chem (2023) 93 : 117441 -117441
PubMed 37586181 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 5.33 6.97

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3134157 1 6.97
CHEMBL4574678 1 6.35
CHEMBL5420859 1 6.04
CHEMBL5409737 1 6.03
CHEMBL5412115 1 5.83
CHEMBL5436330 1 5.11
CHEMBL5396922 1 5.05
CHEMBL5399307 1 4.75
CHEMBL5407736 1 4.66
CHEMBL5432322 1 4.52
CHEMBL5424312 1 4.45
CHEMBL5419726 1 4.18
CHEMBL5407618 1 -
CHEMBL5405542 1 -
CHEMBL5433518 1 -
CHEMBL5411043 1 -
CHEMBL5421314 1 -
CHEMBL5432767 1 -
CHEMBL5414729 1 -
CHEMBL5426621 1 -
CHEMBL5416857 1 -
CHEMBL5439990 1 -
CHEMBL5408050 1 -
CHEMBL5397765 1 -
CHEMBL5407813 1 -
CHEMBL5395822 1 -
CHEMBL5419612 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3134157 IC50 = 107.0 nM 6.97
CHEMBL4574678 IC50 = 446.0 nM 6.35
CHEMBL5420859 IC50 = 917.0 nM 6.04
CHEMBL5409737 IC50 = 924.0 nM 6.03
CHEMBL5412115 IC50 = 1470.0 nM 5.83
CHEMBL5436330 IC50 = 7763.0 nM 5.11
CHEMBL5396922 IC50 = 8963.0 nM 5.05
CHEMBL5399307 IC50 = 17680.0 nM 4.75
CHEMBL5407736 IC50 = 21850.0 nM 4.66
CHEMBL5432322 IC50 = 30430.0 nM 4.52
CHEMBL5424312 IC50 = 35650.0 nM 4.45
CHEMBL5419726 IC50 = 66220.0 nM 4.18
CHEMBL5407618 IC50 - -
CHEMBL5405542 IC50 - -
CHEMBL5433518 IC50 - -
CHEMBL5411043 IC50 - -
CHEMBL5421314 IC50 - -
CHEMBL5432767 IC50 - -
CHEMBL5414729 IC50 - -
CHEMBL5426621 IC50 - -
CHEMBL5416857 IC50 - -
CHEMBL5439990 IC50 - -
CHEMBL5408050 IC50 - -
CHEMBL5397765 IC50 - -
CHEMBL5407813 IC50 - -
CHEMBL5395822 IC50 - -
CHEMBL5419612 IC50 - -