Assay Detail
Binding
CHEMBL5367008
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human NOX4 expressed in HEK293T cells assessed as inhibition of teracycline-induced hydrogen peroxide production pretreated for 18 hrs with tetracycline and treated with compound for 15 mins and measured at 10 mins post-compound treatment by Amplex Red based fluorescence analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
NADPH Oxidases: From Molecular Mechanisms to Current Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.30 | 7.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL397686 | DIPHENYLENEIODONIUM CHLORIDE | — | 1 | 7.05 |
| CHEMBL301982 | CELASTROL | — | 1 | 5.55 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL397686 | DIPHENYLENEIODONIUM CHLORIDE | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL301982 | CELASTROL | IC50 | = | 2790.0 | nM | 5.55 |