Assay Detail
Binding
CHEMBL5368623
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of NanoLuc-fused VHL (unknown origin) transfected in live HEK293 cells using NanoGlo substrate incubated for 30 mins by tracer based NanoBRET assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
von Hippel-Lindau disease tumor suppressor (CHEMBL3108660) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Expanding the Structural Diversity at the Phenylene Core of Ligands for the von Hippel-Lindau E3 Ubiquitin Ligase: Development of Highly Potent Hypoxia-Inducible Factor-1α Stabilizers.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 6.46 | 6.79 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5421220 | — | — | 1 | 6.79 |
| CHEMBL5425778 | — | — | 1 | 6.50 |
| CHEMBL5403921 | — | — | 1 | 6.44 |
| CHEMBL4225537 | — | — | 1 | 6.12 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5421220 | — | IC50 | = | 164.0 | nM | 6.79 |
| CHEMBL5425778 | — | IC50 | = | 320.0 | nM | 6.50 |
| CHEMBL5403921 | — | IC50 | = | 366.0 | nM | 6.44 |
| CHEMBL4225537 | — | IC50 | = | 750.0 | nM | 6.12 |