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Assay Detail

CHEMBL5369253

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to CDK2/Cyclin E1 (unknown origin) using Ulight-MBP as substrate assessed as inhibition constant preincubated for 30 mins followed by substrate addition measured after 90 mins in presence of ATP by TR-FRET assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of Selective Tertiary Amide Inhibitors of Cyclin-Dependent Kinase 2 (CDK2).
Zeng M, Grandner JM, Bryan MC, Verma V, Larouche-Gauthier R, Leclerc JP, Zhao L, Haghshenas P, Aubert-Nicol S, Yadav A, Ashley M, Chen JZ, Durk M, Samy KE, Nespi M, Levy E, Merrick K, Moffat JG, Murray J, Oh A, Orr C, Segal E, Sims J, Sneeringer C, Prangley M, Vartanian S, Magnuson S, Parr BT.
ACS Med Chem Lett (2023) 14 : 1179 -1187
PubMed 37736184 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 31 8.57 9.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4446357 EBVACICLIB 2.0 1 9.92
CHEMBL5402601 1 9.89
CHEMBL5434470 1 9.80
CHEMBL5434780 1 9.77
CHEMBL5422841 1 9.74
CHEMBL5423838 1 9.74
CHEMBL5405526 1 9.74
CHEMBL5399918 1 9.68
CHEMBL5415273 1 9.47
CHEMBL5425198 1 9.36
CHEMBL5422107 1 9.31
CHEMBL5398255 1 9.20
CHEMBL5402758 1 9.00
CHEMBL5433947 1 8.96
CHEMBL5408374 1 8.96
CHEMBL5201870 TEGTOCICLIB 2.0 1 8.85
CHEMBL5429216 1 8.68
CHEMBL3655762 CYC-065 2.0 1 8.64
CHEMBL5428299 1 8.19
CHEMBL5399703 1 8.10
CHEMBL5440034 1 8.06
CHEMBL5403871 1 8.05
CHEMBL4112022 1 7.96
CHEMBL5401047 1 7.89
CHEMBL3298678 1 7.80
CHEMBL5427628 1 7.77
CHEMBL5409832 1 7.15
CHEMBL5404558 1 7.06
CHEMBL5438013 1 6.62
CHEMBL5403389 1 6.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4446357 EBVACICLIB Ki = 0.12 nM 9.92
CHEMBL5402601 Ki = 0.13 nM 9.89
CHEMBL5434470 Ki = 0.16 nM 9.80
CHEMBL5434780 Ki = 0.17 nM 9.77
CHEMBL5422841 Ki = 0.18 nM 9.74
CHEMBL5423838 Ki = 0.18 nM 9.74
CHEMBL5405526 Ki = 0.18 nM 9.74
CHEMBL5399918 Ki = 0.21 nM 9.68
CHEMBL5415273 Ki = 0.34 nM 9.47
CHEMBL5425198 Ki = 0.44 nM 9.36
CHEMBL5422107 Ki = 0.49 nM 9.31
CHEMBL5398255 Ki = 0.63 nM 9.20
CHEMBL5402758 Ki = 1.0 nM 9.00
CHEMBL5433947 Ki = 1.1 nM 8.96
CHEMBL5408374 Ki = 1.1 nM 8.96
CHEMBL5201870 TEGTOCICLIB Ki = 1.4 nM 8.85
CHEMBL5429216 Ki = 2.1 nM 8.68
CHEMBL3655762 CYC-065 Ki = 2.3 nM 8.64
CHEMBL5428299 Ki = 6.5 nM 8.19
CHEMBL5399703 Ki = 7.9 nM 8.10
CHEMBL5440034 Ki = 8.7 nM 8.06
CHEMBL5403871 Ki = 9.0 nM 8.05
CHEMBL4112022 Ki = 11.0 nM 7.96
CHEMBL5401047 Ki = 13.0 nM 7.89
CHEMBL3298678 Ki = 16.0 nM 7.80
CHEMBL5427628 Ki = 17.0 nM 7.77
CHEMBL5409832 Ki = 71.0 nM 7.15
CHEMBL5404558 Ki = 88.0 nM 7.06
CHEMBL5438013 Ki = 240.0 nM 6.62
CHEMBL5403389 Ki = 750.0 nM 6.12
CHEMBL5428634 Ki = 920.0 nM 6.04