Assay Detail
Binding
CHEMBL5371673
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to BRAF V600E mutant in human WM278 cells by KINOMEscann Kinase binding assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | WM278 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase B-raf (CHEMBL5145) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
A cyanine-based NIR fluorescent Vemurafenib analog to probe BRAF<sup>V600E</sup> in cancer cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 3 | 8.15 | 8.55 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5440394 | — | — | 1 | 8.55 |
| CHEMBL1229517 | VEMURAFENIB | 4.0 | 1 | 8.00 |
| CHEMBL5419570 | — | — | 1 | 7.89 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5440394 | — | Kd | = | 2.8 | nM | 8.55 |
| CHEMBL1229517 | VEMURAFENIB | Kd | = | 10.0 | nM | 8.00 |
| CHEMBL5419570 | — | Kd | = | 13.0 | nM | 7.89 |