Assay Detail
Functional
CHEMBL5371676
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Cytotoxicity against human HT-29 cells expressing heterozygous BRAF V600E mutant assessed as cell growth measured after 7 days of treatment by crystal violet assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HT-29 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
A cyanine-based NIR fluorescent Vemurafenib analog to probe BRAF<sup>V600E</sup> in cancer cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 7.12 | 7.57 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5440394 | — | — | 1 | 7.57 |
| CHEMBL1229517 | VEMURAFENIB | 4.0 | 1 | 7.31 |
| CHEMBL5419570 | — | — | 1 | 6.47 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5440394 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL1229517 | VEMURAFENIB | IC50 | = | 49.0 | nM | 7.31 |
| CHEMBL5419570 | — | IC50 | = | 340.0 | nM | 6.47 |