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Assay Detail

CHEMBL5371979

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IDH1 R132C mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as NADPH consumption using alpha-KG as substrate measured after 60 mins
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based discovery of IHMT-IDH1-053 as a potent irreversible IDH1 mutant selective inhibitor.
Liang Q, Wang B, Zou F, Guo G, Wang W, Wang W, Liu Q, Shen L, Hu C, Wang W, Wang A, Huang T, He Y, Xia R, Ge J, Liu J, Liu Q.
Eur J Med Chem (2023) 256 : 115411 -115411
PubMed 37209613 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.29 7.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3989958 IVOSIDENIB 4.0 1 7.72
CHEMBL5425749 1 6.86

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3989958 IVOSIDENIB IC50 = 19.0 nM 7.72
CHEMBL5425749 IC50 = 139.0 nM 6.86