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Assay Detail

CHEMBL5373000

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of Biotin-p53K381acK382me2 from N-terminal his6-tagged 53BP1 (1484 to 1603 residues) (unknown origin) expressed in Rosetta2 BL21 (DE3) pLysS competent cells by TR-FRET assay
37
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

TP53-binding protein 1 (CHEMBL2424509)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a 53BP1 Small Molecule Antagonist Using a Focused DNA-Encoded Library Screen.
Shell DJ, Foley CA, Wang Q, Smith CM, Guduru SKR, Zeng H, Dong A, Norris-Drouin JL, Axtman M, Hardy PB, Gupta G, Halabelian L, Frye SV, James LI, Pearce KH.
J Med Chem (2023) 66 : 14133 -14149
PubMed 37782247 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 5.17 6.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5439383 1 6.34
CHEMBL5403027 1 6.33
CHEMBL5402301 1 6.07
CHEMBL5431269 1 5.89
CHEMBL5433157 1 5.85
CHEMBL5415556 1 5.72
CHEMBL5423332 1 5.66
CHEMBL5440895 1 5.47
CHEMBL5421180 1 5.42
CHEMBL5430714 1 5.39
CHEMBL5440393 1 5.30
CHEMBL5397384 1 5.25
CHEMBL5400477 1 5.24
CHEMBL5397306 1 5.23
CHEMBL5395836 1 5.18
CHEMBL5428966 1 5.17
CHEMBL5429915 1 5.13
CHEMBL5412662 1 5.11
CHEMBL5418978 1 5.09
CHEMBL5438168 1 5.00
CHEMBL5398002 1 5.00
CHEMBL5409275 1 4.96
CHEMBL5437926 1 4.92
CHEMBL5439940 1 4.85
CHEMBL5416576 1 4.82
CHEMBL5440782 1 4.82
CHEMBL5423187 1 4.75
CHEMBL5431260 1 4.66
CHEMBL5431898 1 4.55
CHEMBL5412159 1 4.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5439383 IC50 = 460.0 nM 6.34
CHEMBL5403027 IC50 = 470.0 nM 6.33
CHEMBL5402301 IC50 = 860.0 nM 6.07
CHEMBL5431269 IC50 = 1300.0 nM 5.89
CHEMBL5433157 IC50 = 1400.0 nM 5.85
CHEMBL5415556 IC50 = 1900.0 nM 5.72
CHEMBL5423332 IC50 = 2200.0 nM 5.66
CHEMBL5440895 IC50 = 3400.0 nM 5.47
CHEMBL5421180 IC50 = 3800.0 nM 5.42
CHEMBL5430714 IC50 = 4100.0 nM 5.39
CHEMBL5440393 IC50 = 5000.0 nM 5.30
CHEMBL5397384 IC50 = 5600.0 nM 5.25
CHEMBL5400477 IC50 = 5700.0 nM 5.24
CHEMBL5397306 IC50 = 5900.0 nM 5.23
CHEMBL5395836 IC50 = 6600.0 nM 5.18
CHEMBL5428966 IC50 = 6800.0 nM 5.17
CHEMBL5429915 IC50 = 7400.0 nM 5.13
CHEMBL5412662 IC50 = 7800.0 nM 5.11
CHEMBL5418978 IC50 = 8100.0 nM 5.09
CHEMBL5398002 IC50 = 10000.0 nM 5.00
CHEMBL5438168 IC50 = 10000.0 nM 5.00
CHEMBL5409275 IC50 = 11000.0 nM 4.96
CHEMBL5437926 IC50 = 12000.0 nM 4.92
CHEMBL5439940 IC50 = 14000.0 nM 4.85
CHEMBL5416576 IC50 = 15000.0 nM 4.82
CHEMBL5440782 IC50 = 15000.0 nM 4.82
CHEMBL5423187 IC50 = 18000.0 nM 4.75
CHEMBL5431260 IC50 = 22000.0 nM 4.66
CHEMBL5431898 IC50 = 28000.0 nM 4.55
CHEMBL5412159 IC50 = 32000.0 nM 4.50
CHEMBL5420747 IC50 = 38000.0 nM 4.42
CHEMBL5416659 IC50 = 44000.0 nM 4.36
CHEMBL5434158 IC50 = 83000.0 nM 4.08
CHEMBL5419460 IC50 > 33000.0 nM -
CHEMBL5406735 IC50 > 100000.0 nM -
CHEMBL5441134 IC50 > 100000.0 nM -
CHEMBL5414804 IC50 > 100000.0 nM -