Assay Detail
Binding
CHEMBL5377322
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Inhibition of Fam-labeled BimBH3 peptide binding to wild type N-terminal 6His-tagged human recombinant HSP70 NBD (1 to 383 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant incubated for 24 hrs by fluorescence polarization assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Exploiting the "Hot-Spots" of Hsp70<b>-</b>Bim Protein<b>-</b>Protein Interaction to Optimize the 1-Oxo-1<i>H</i>-phenalene-2,3-dicarbonitrile Analogues as Specific Hsp70<b>-</b>Bim Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 1 | 6.68 | 6.68 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5397919 | — | — | 1 | 6.68 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5397919 | — | Kd | = | 210.0 | nM | 6.68 |