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Assay Detail

CHEMBL5381014

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Partial agonist activity at D2LR-Nluc in HEK293T cells assessed as mGsi-Venus recruitment using coelenterazine h as substrate pretreated with substrate followed by compound addition measured after 10 mins by BRET analysis
22
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(2) dopamine receptor (CHEMBL217)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dopamine D<sub>3</sub>/D<sub>2</sub> Receptor Ligands Based on Cariprazine for the Treatment of Psychostimulant Use Disorders That May Be Dual Diagnosed with Affective Disorders.
Gogarnoiu ES, Vogt CD, Sanchez J, Bonifazi A, Saab E, Shaik AB, Soler-Cedeño O, Bi GH, Klein B, Xi ZX, Lane JR, Newman AH.
J Med Chem (2023) 66 : 1809 -1834
PubMed 36661568 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 22 7.98 8.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2028019 CARIPRAZINE 4.0 2 8.87
CHEMBL5431103 2 8.65
CHEMBL5427695 2 8.39
CHEMBL5437660 2 8.15
CHEMBL5438081 2 8.00
CHEMBL5403268 2 7.96
CHEMBL5400528 2 7.93
CHEMBL5435745 2 7.66
CHEMBL5424897 2 7.58
CHEMBL240773 QUINPIROLE 2.0 2 7.40
CHEMBL59 DOPAMINE 4.0 2 7.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2028019 CARIPRAZINE EC50 = 1.34 nM 8.87
CHEMBL2028019 CARIPRAZINE EC50 = 1.349 nM 8.87
CHEMBL5431103 EC50 = 2.239 nM 8.65
CHEMBL5431103 EC50 = 2.26 nM 8.65
CHEMBL5427695 EC50 = 4.074 nM 8.39
CHEMBL5427695 EC50 = 4.1 nM 8.39
CHEMBL5437660 EC50 = 7.079 nM 8.15
CHEMBL5437660 EC50 = 7.08 nM 8.15
CHEMBL5438081 EC50 = 10.0 nM 8.00
CHEMBL5438081 EC50 = 9.92 nM 8.00
CHEMBL5403268 EC50 = 11.02 nM 7.96
CHEMBL5403268 EC50 = 10.96 nM 7.96
CHEMBL5400528 EC50 = 11.75 nM 7.93
CHEMBL5400528 EC50 = 11.86 nM 7.93
CHEMBL5435745 EC50 = 21.88 nM 7.66
CHEMBL5435745 EC50 = 21.85 nM 7.66
CHEMBL5424897 EC50 = 26.3 nM 7.58
CHEMBL5424897 EC50 = 26.21 nM 7.58
CHEMBL240773 QUINPIROLE EC50 = 39.81 nM 7.40
CHEMBL240773 QUINPIROLE EC50 = 39.78 nM 7.40
CHEMBL59 DOPAMINE EC50 = 66.07 nM 7.18
CHEMBL59 DOPAMINE EC50 = 66.25 nM 7.18