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Assay Detail

CHEMBL5381016

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at D3 receptor in HEK293T cells assessed as G beta-gamma release from G alpha oA using coelenterazine h as substrate pretreated with substrate followed by compound addition measured after 10 mins by BRET analysis
16
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(3) dopamine receptor (CHEMBL234)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dopamine D<sub>3</sub>/D<sub>2</sub> Receptor Ligands Based on Cariprazine for the Treatment of Psychostimulant Use Disorders That May Be Dual Diagnosed with Affective Disorders.
Gogarnoiu ES, Vogt CD, Sanchez J, Bonifazi A, Saab E, Shaik AB, Soler-Cedeño O, Bi GH, Klein B, Xi ZX, Lane JR, Newman AH.
J Med Chem (2023) 66 : 1809 -1834
PubMed 36661568 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 8.39 8.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5431103 2 8.80
CHEMBL5427695 2 8.79
CHEMBL2028019 CARIPRAZINE 4.0 2 8.72
CHEMBL240773 QUINPIROLE 2.0 2 8.59
CHEMBL5403268 2 8.54
CHEMBL5400528 2 8.22
CHEMBL5438081 2 7.80
CHEMBL5435745 2 7.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5431103 EC50 = 1.585 nM 8.80
CHEMBL5431103 EC50 = 1.6 nM 8.80
CHEMBL5427695 EC50 = 1.622 nM 8.79
CHEMBL5427695 EC50 = 1.64 nM 8.79
CHEMBL2028019 CARIPRAZINE EC50 = 1.905 nM 8.72
CHEMBL2028019 CARIPRAZINE EC50 = 1.89 nM 8.72
CHEMBL240773 QUINPIROLE EC50 = 2.57 nM 8.59
CHEMBL240773 QUINPIROLE EC50 = 2.59 nM 8.59
CHEMBL5403268 EC50 = 2.884 nM 8.54
CHEMBL5403268 EC50 = 2.87 nM 8.54
CHEMBL5400528 EC50 = 6.026 nM 8.22
CHEMBL5400528 EC50 = 6.07 nM 8.22
CHEMBL5438081 EC50 = 15.85 nM 7.80
CHEMBL5438081 EC50 = 16.02 nM 7.79
CHEMBL5435745 EC50 = 19.95 nM 7.70
CHEMBL5435745 EC50 = 19.91 nM 7.70