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Assay Detail

CHEMBL5384101

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inverse agonist activity at human DOR expressed in human HTLA cells assessed as beta arrestin-2 recruitment by ONE-Glo luciferase assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HTLA
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Delta-type opioid receptor (CHEMBL236)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Binding Mechanism of Pyrazoloisoquinoline-Based Novel β-Arrestin Inverse Agonists of the Kappa-Opioid Receptor.
Jung JH, Jang IH, Yang MY, Kim S, Kim SK, Goddard WA, Kim YC.
J Med Chem (2023) 66 : 5154 -5170
PubMed 36987735 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 6.62 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5439465 1 7.30
CHEMBL5430894 1 6.68
CHEMBL5395170 1 6.32
CHEMBL5429274 1 6.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5439465 EC50 = 49.5 nM 7.30
CHEMBL5430894 EC50 = 207.0 nM 6.68
CHEMBL5395170 EC50 = 483.0 nM 6.32
CHEMBL5429274 EC50 = 691.0 nM 6.16