Assay Detail
Binding
CHEMBL5385443
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of HIV-1 reverse transcriptase RT52A mutant using ABTS as substrate preincubated with compound for 1 hr followed by template-primer addition for 1 hr and measured 20 mins after substrate addition by absorbance based Roche colorimetric assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Human immunodeficiency virus 1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Human immunodeficiency virus type 1 reverse transcriptase (CHEMBL247) ↗| Type | SINGLE PROTEIN |
| Organism | Human immunodeficiency virus 1 |
Publication
Halo Library, a Tool for Rapid Identification of Ligand Binding Sites on Proteins Using Crystallographic Fragment Screening.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 2.63 | 2.68 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5413115 | — | — | 1 | 2.68 |
| CHEMBL452901 | — | — | 1 | 2.68 |
| CHEMBL23507 | 5-FLUOROINDOLE-2-CARBOXYLIC ACID | — | 1 | 2.54 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5413115 | — | IC50 | = | 2100000.0 | nM | 2.68 |
| CHEMBL452901 | — | IC50 | = | 2100000.0 | nM | 2.68 |
| CHEMBL23507 | 5-FLUOROINDOLE-2-CARBOXYLIC ACID | IC50 | = | 2900000.0 | nM | 2.54 |