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Assay Detail

CHEMBL5385450

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant N-terminal IgG tagged and C-terminal His tagged C1S (358 to 688 aa residues) expressed in HEK293 cells using Z-Lys-SBzl peptide as substrate pretreated with compound followed by substrate addition incubated for 60 mins
20
Total Activities
20
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Complement C1s subcomponent (CHEMBL3913)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Novel Series of Potent, Selective, Orally Available, and Brain-Penetrable C1s Inhibitors for Modulation of the Complement Pathway.
Ikeda Z, Kamei T, Sasaki Y, Reynolds M, Sakai N, Yoshikawa M, Tawada M, Morishita N, Dougan DR, Chen CH, Levin I, Zou H, Kuno M, Arimura N, Kikukawa Y, Kondo M, Tohyama K, Sato K.
J Med Chem (2023) 66 : 6354 -6371
PubMed 37120845 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 6.05 8.15
INH 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5435382 1 8.15
CHEMBL5417464 1 7.44
CHEMBL5418327 1 6.77
CHEMBL5407328 1 6.43
CHEMBL5410431 1 6.41
CHEMBL5418278 1 6.25
CHEMBL5437582 1 6.24
CHEMBL5432292 1 6.09
CHEMBL5398476 1 5.92
CHEMBL5439103 1 5.85
CHEMBL5437882 1 5.85
CHEMBL5411534 1 5.60
CHEMBL5398549 1 5.38
CHEMBL5411177 1 5.29
CHEMBL5412594 1 5.17
CHEMBL5410965 1 4.02
CHEMBL5424714 1 -
CHEMBL5431053 1 -
CHEMBL5413734 1 -
CHEMBL5418024 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5435382 IC50 = 7.0 nM 8.15
CHEMBL5417464 IC50 = 36.0 nM 7.44
CHEMBL5418327 IC50 = 170.0 nM 6.77
CHEMBL5407328 IC50 = 370.0 nM 6.43
CHEMBL5410431 IC50 = 390.0 nM 6.41
CHEMBL5418278 IC50 = 560.0 nM 6.25
CHEMBL5437582 IC50 = 580.0 nM 6.24
CHEMBL5432292 IC50 = 820.0 nM 6.09
CHEMBL5398476 IC50 = 1200.0 nM 5.92
CHEMBL5439103 IC50 = 1400.0 nM 5.85
CHEMBL5437882 IC50 = 1400.0 nM 5.85
CHEMBL5411534 IC50 = 2500.0 nM 5.60
CHEMBL5398549 IC50 = 4200.0 nM 5.38
CHEMBL5411177 IC50 = 5100.0 nM 5.29
CHEMBL5412594 IC50 = 6700.0 nM 5.17
CHEMBL5410965 IC50 = 96000.0 nM 4.02
CHEMBL5424714 IC50 > 30000.0 nM -
CHEMBL5431053 INH = 4.5 uM -
CHEMBL5413734 IC50 > 300000.0 nM -
CHEMBL5418024 IC50 > 300000.0 nM -