Assay Detail
Binding
CHEMBL5388995
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of fluorescent probe (R)-(+)-ethyl 5-amino2-methyl-1,2-dihydro-3-phenylpyrido[3,4-b]pyrazin-7-ylcarbamate from calf brain tubulin colchicine binding site assessed as dissociation constant at 0.05 to 70 uM incubated for 30 mins by fluorescence based assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Bos taurus |
| Confidence | 5 — Multiple protein complex / RNA |
| Curated By | Autocuration |
Publication
PM534, an Optimized Target-Protein Interaction Strategy through the Colchicine Site of Tubulin.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 2 | 6.86 | 7.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5437532 | — | — | 1 | 7.70 |
| CHEMBL5418923 | — | — | 1 | 6.02 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5437532 | — | Kd | = | 20.0 | nM | 7.70 |
| CHEMBL5418923 | — | Kd | = | 960.0 | nM | 6.02 |