Assay Detail
Functional
CHEMBL5393232
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Antiproliferative activity against chinese hamster R2 cells expressing human PCFT4 assessed as inhibition of cell growth incubated for 96 hrs by CellTiter-Blue cell viability assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Cricetulus griseus |
| Cell Type | R2 |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Discovery of Tumor-Targeted 6-Methyl Substituted Pemetrexed and Related Antifolates with Selective Loss of RFC Transport.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 7.35 | 8.12 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5433810 | — | — | 1 | 8.12 |
| CHEMBL225072 | PEMETREXED | 4.0 | 1 | 7.65 |
| CHEMBL5416929 | — | — | 1 | 7.59 |
| CHEMBL5398059 | — | — | 1 | 7.29 |
| CHEMBL5417031 | — | — | 1 | 7.19 |
| CHEMBL3407519 | — | — | 1 | 7.10 |
| CHEMBL374172 | — | — | 1 | 6.48 |
| CHEMBL5404618 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5433810 | — | IC50 | = | 7.66 | nM | 8.12 |
| CHEMBL225072 | PEMETREXED | IC50 | = | 22.26 | nM | 7.65 |
| CHEMBL5416929 | — | IC50 | = | 25.9 | nM | 7.59 |
| CHEMBL5398059 | — | IC50 | = | 51.46 | nM | 7.29 |
| CHEMBL5417031 | — | IC50 | = | 64.69 | nM | 7.19 |
| CHEMBL3407519 | — | IC50 | = | 80.2 | nM | 7.10 |
| CHEMBL374172 | — | IC50 | = | 329.0 | nM | 6.48 |
| CHEMBL5404618 | — | IC50 | > | 1000.0 | nM | - |