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Assay Detail

CHEMBL5442207

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
PDSP Secondary Binding target: TSPO - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Category PDSP assay
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Translocator protein (CHEMBL5742)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

EUbOPEN Chemogenomics Library - PDSP Secondary Binding
EUbOPEN
(2024)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 30 5.89 6.95

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3716980 1 6.95
CHEMBL428504 1 6.74
CHEMBL426559 LAROPIPRANT 4.0 1 6.54
CHEMBL1766103 1 6.28
CHEMBL2110363 1 6.10
CHEMBL256470 1 6.10
CHEMBL2435402 1 6.09
CHEMBL405355 NIGULDIPINE 2.0 1 6.02
CHEMBL2030155 1 6.01
CHEMBL21283 1 6.01
CHEMBL487553 1 6.01
CHEMBL540612 1 5.93
CHEMBL2181226 1 5.89
CHEMBL3086703 1 5.86
CHEMBL23327 PURVALANOLA 1 5.82
CHEMBL5087624 1 5.79
CHEMBL3342358 1 5.78
CHEMBL475903 1 5.73
CHEMBL3410223 1 5.71
CHEMBL1200492 NEFAZODONE HYDROCHLORIDE 4.0 1 5.70
CHEMBL2103873 MACITENTAN 4.0 1 5.67
CHEMBL38182 1 5.52
CHEMBL225155 1 5.51
CHEMBL2386353 1 5.48
CHEMBL9506 DEVAZEPIDE 2.0 1 5.48
CHEMBL531742 1 5.40
CHEMBL3545181 TIOTROPIUM BROMIDE 4.0 1 5.37
CHEMBL5077459 1 5.32
CHEMBL1472 PROTIRELIN 4.0 1 -
CHEMBL1558 PRAZOSIN HYDROCHLORIDE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3716980 Ki = 111.12 nM 6.95
CHEMBL428504 Ki = 183.97 nM 6.74
CHEMBL426559 LAROPIPRANT Ki = 286.42 nM 6.54
CHEMBL1766103 Ki = 522.4 nM 6.28
CHEMBL2110363 Ki = 789.49 nM 6.10
CHEMBL256470 Ki = 799.1 nM 6.10
CHEMBL2435402 Ki = 802.97 nM 6.09
CHEMBL405355 NIGULDIPINE Ki = 952.9 nM 6.02
CHEMBL2030155 Ki = 969.17 nM 6.01
CHEMBL21283 Ki = 974.88 nM 6.01
CHEMBL487553 Ki = 968.95 nM 6.01
CHEMBL540612 Ki = 1179.92 nM 5.93
CHEMBL2181226 Ki = 1301.66 nM 5.89
CHEMBL3086703 Ki = 1378.79 nM 5.86
CHEMBL23327 PURVALANOLA Ki = 1515.83 nM 5.82
CHEMBL5087624 Ki = 1631.92 nM 5.79
CHEMBL3342358 Ki = 1662.07 nM 5.78
CHEMBL475903 Ki = 1879.32 nM 5.73
CHEMBL3410223 Ki = 1951.86 nM 5.71
CHEMBL1200492 NEFAZODONE HYDROCHLORIDE Ki = 1986.32 nM 5.70
CHEMBL2103873 MACITENTAN Ki = 2118.36 nM 5.67
CHEMBL38182 Ki = 3028.31 nM 5.52
CHEMBL225155 Ki = 3115.3 nM 5.51
CHEMBL2386353 Ki = 3298.37 nM 5.48
CHEMBL9506 DEVAZEPIDE Ki = 3332.73 nM 5.48
CHEMBL531742 Ki = 4012.36 nM 5.40
CHEMBL3545181 TIOTROPIUM BROMIDE Ki = 4286.47 nM 5.37
CHEMBL5077459 Ki = 4781.34 nM 5.32
CHEMBL1472 PROTIRELIN Ki > 10000.0 nM -
CHEMBL1558 PRAZOSIN HYDROCHLORIDE Ki > 10000.0 nM -