Assay Detail
Binding
CHEMBL5442216
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: HTR3A - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 12 | 6.14 | 7.20 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4743049 | — | — | 1 | 7.20 |
| CHEMBL2164327 | — | — | 1 | 6.58 |
| CHEMBL1633 | KETOTIFEN FUMARATE | 4.0 | 1 | 6.47 |
| CHEMBL519240 | — | — | 1 | 6.45 |
| CHEMBL3675743 | — | — | 1 | 6.44 |
| CHEMBL5075931 | — | — | 1 | 6.18 |
| CHEMBL542139 | — | — | 1 | 6.00 |
| CHEMBL1256998 | — | — | 1 | 5.88 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 5.82 |
| CHEMBL59898 | — | — | 1 | 5.78 |
| CHEMBL543113 | — | — | 1 | 5.68 |
| CHEMBL1516474 | TEGASEROD MALEATE | 4.0 | 1 | 5.23 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4743049 | — | Ki | = | 63.69 | nM | 7.20 |
| CHEMBL2164327 | — | Ki | = | 260.79 | nM | 6.58 |
| CHEMBL1633 | KETOTIFEN FUMARATE | Ki | = | 338.93 | nM | 6.47 |
| CHEMBL519240 | — | Ki | = | 351.84 | nM | 6.45 |
| CHEMBL3675743 | — | Ki | = | 360.29 | nM | 6.44 |
| CHEMBL5075931 | — | Ki | = | 661.83 | nM | 6.18 |
| CHEMBL542139 | — | Ki | = | 988.1 | nM | 6.00 |
| CHEMBL1256998 | — | Ki | = | 1307.37 | nM | 5.88 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 1528.1 | nM | 5.82 |
| CHEMBL59898 | — | Ki | = | 1662.65 | nM | 5.78 |
| CHEMBL543113 | — | Ki | = | 2066.81 | nM | 5.68 |
| CHEMBL1516474 | TEGASEROD MALEATE | Ki | = | 5843.86 | nM | 5.23 |