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Assay Detail

CHEMBL5445895

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Affinity Phenotypic Cellular interaction: (Immunoblotting (phosphorylation of S6K1 Thr-389 in p53xe2x88x92/xe2x88x92 MEFs cells)) EUB0000092b MTOR
1
Total Activities
1
Compounds Tested
1
Activity Types
3
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type MEF
Category Affinity phenotypic cellular assay
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase mTOR (CHEMBL2842)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Affinity Phenotypic Cellular Literature for EUbOPEN Chemogenomic Library
EUbOPEN
(2024)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.70 8.70

Assay Parameters

Parameter Type Value Units Text
Mode of action Mode of action - Inhibitor
Protein family Protein family - Protein kinase
Recommended concentration Recommended concentration 100.0 nM

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1256459 TORIN1 1 8.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1256459 TORIN1 IC50 = 2.0 nM 8.70