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Assay Detail

CHEMBL5445985

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Affinity On-target Cellular interaction: (Cellular mechanistic assay (measuring phosphorylation of INSR in NIH-3T3-hIGF-1R and NIH-3T3-hIR cell lines, detection and quantifying of Eu-fluorescence)) EUB0000601a INSR
1
Total Activities
1
Compounds Tested
1
Activity Types
3
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type NIH3T3
Category Affinity on-target cellular assay
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Insulin receptor (CHEMBL1981)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Affinity On-target Cellular Literature for EUbOPEN Chemogenomic Library
EUbOPEN
(2024)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.72 7.72

Assay Parameters

Parameter Type Value Units Text
Mode of action Mode of action - Inhibitor
Protein family Protein family - Protein Kinase
Recommended concentration Recommended concentration 100.0 nM

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL466397 1 7.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL466397 IC50 = 19.0 nM 7.72