Assay Detail
Binding
CHEMBL5500660
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of fluorescent tracer 3'-chloro-N-(2-(3-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)thioureido)ethyl)-4'-(alpha-D-mannopyranosyloxy)biphenyl-4-carboxamide from Escherichia coli F18 FimH lectin domain expressed in Escherichia coli HM125 assessed as dissociation constant incubated for 24 hrs by competitive binding based fluorescence polarization assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Does size matter? - Comparing pyranoses with septanoses as ligands of the bacterial lectin FimH.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 19 | 6.65 | 9.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5556130 | — | — | 1 | 9.52 |
| CHEMBL3417101 | — | — | 1 | 9.30 |
| CHEMBL4085991 | — | — | 1 | 8.55 |
| CHEMBL1170072 | — | — | 1 | 7.82 |
| CHEMBL1170455 | — | — | 1 | 7.55 |
| CHEMBL5505945 | — | — | 1 | 7.22 |
| CHEMBL5512541 | — | — | 1 | 7.00 |
| CHEMBL5556815 | — | — | 1 | 6.92 |
| CHEMBL5558248 | — | — | 1 | 6.58 |
| CHEMBL5557754 | — | — | 1 | 5.89 |
| CHEMBL5505793 | — | — | 1 | 5.84 |
| CHEMBL5556913 | — | — | 1 | 5.84 |
| CHEMBL5527985 | — | — | 1 | 5.80 |
| CHEMBL5555901 | — | — | 1 | 5.71 |
| CHEMBL5505893 | — | — | 1 | 5.57 |
| CHEMBL5532476 | — | — | 1 | 5.25 |
| CHEMBL5532605 | — | — | 1 | 5.15 |
| CHEMBL5518062 | — | — | 1 | 4.10 |
| CHEMBL5512490 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5556130 | — | Kd | = | 0.3 | nM | 9.52 |
| CHEMBL3417101 | — | Kd | = | 0.5 | nM | 9.30 |
| CHEMBL4085991 | — | Kd | = | 2.8 | nM | 8.55 |
| CHEMBL1170072 | — | Kd | = | 15.1 | nM | 7.82 |
| CHEMBL1170455 | — | Kd | = | 28.3 | nM | 7.55 |
| CHEMBL5505945 | — | Kd | = | 60.0 | nM | 7.22 |
| CHEMBL5512541 | — | Kd | = | 100.0 | nM | 7.00 |
| CHEMBL5556815 | — | Kd | = | 120.0 | nM | 6.92 |
| CHEMBL5558248 | — | Kd | = | 260.0 | nM | 6.58 |
| CHEMBL5557754 | — | Kd | = | 1300.0 | nM | 5.89 |
| CHEMBL5505793 | — | Kd | = | 1440.0 | nM | 5.84 |
| CHEMBL5556913 | — | Kd | = | 1440.0 | nM | 5.84 |
| CHEMBL5527985 | — | Kd | = | 1580.0 | nM | 5.80 |
| CHEMBL5555901 | — | Kd | = | 1940.0 | nM | 5.71 |
| CHEMBL5505893 | — | Kd | = | 2720.0 | nM | 5.57 |
| CHEMBL5532476 | — | Kd | = | 5670.0 | nM | 5.25 |
| CHEMBL5532605 | — | Kd | = | 7050.0 | nM | 5.15 |
| CHEMBL5518062 | — | Kd | = | 80120.0 | nM | 4.10 |
| CHEMBL5512490 | — | Kd | - | — | - |