Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5500841

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HPK1 (unknown origin) using His-tagged SLP-76 as substrate preincubated for 30 mins followed by substrate and ATP addition and measured after 60 mins by TR-FRET assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of 5-aminopyrido[2,3-d]pyrimidin-7(8H)-one derivatives as new hematopoietic progenitor kinase 1 (HPK1) inhibitors.
Qiu X, Liu R, Ling H, Zhou Y, Ren X, Zhou F, Zhang J, Huang W, Wang Z, Ding K.
Eur J Med Chem (2024) 269 : 116310 -116310
PubMed 38479166 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 8.23 9.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5505932 1 9.49
CHEMBL5556074 1 9.44
CHEMBL5512546 1 9.37
CHEMBL4878586 1 8.92
CHEMBL5558035 1 8.84
CHEMBL5558023 1 8.68
CHEMBL5517999 1 8.61
CHEMBL5556510 1 8.47
CHEMBL5555827 1 8.44
CHEMBL5542535 1 8.40
CHEMBL5556731 1 8.39
CHEMBL5556350 1 8.32
CHEMBL5505883 1 8.20
CHEMBL5505790 1 8.12
CHEMBL5558220 1 8.07
CHEMBL5559273 1 8.01
CHEMBL5517850 1 7.96
CHEMBL5505808 1 7.80
CHEMBL5512580 1 7.77
CHEMBL5527890 1 7.65
CHEMBL5559247 1 7.65
CHEMBL5517756 1 7.64
CHEMBL5559387 1 7.36
CHEMBL5537094 1 7.14
CHEMBL5558221 1 7.13
CHEMBL5517774 1 -
CHEMBL5549893 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5505932 IC50 = 0.32 nM 9.49
CHEMBL5556074 IC50 = 0.36 nM 9.44
CHEMBL5512546 IC50 = 0.43 nM 9.37
CHEMBL4878586 IC50 = 1.21 nM 8.92
CHEMBL5558035 IC50 = 1.45 nM 8.84
CHEMBL5558023 IC50 = 2.08 nM 8.68
CHEMBL5517999 IC50 = 2.48 nM 8.61
CHEMBL5556510 IC50 = 3.39 nM 8.47
CHEMBL5555827 IC50 = 3.62 nM 8.44
CHEMBL5542535 IC50 = 3.98 nM 8.40
CHEMBL5556731 IC50 = 4.05 nM 8.39
CHEMBL5556350 IC50 = 4.79 nM 8.32
CHEMBL5505883 IC50 = 6.33 nM 8.20
CHEMBL5505790 IC50 = 7.52 nM 8.12
CHEMBL5558220 IC50 = 8.53 nM 8.07
CHEMBL5559273 IC50 = 9.81 nM 8.01
CHEMBL5517850 IC50 = 10.96 nM 7.96
CHEMBL5505808 IC50 = 15.86 nM 7.80
CHEMBL5512580 IC50 = 16.93 nM 7.77
CHEMBL5527890 IC50 = 22.34 nM 7.65
CHEMBL5559247 IC50 = 22.45 nM 7.65
CHEMBL5517756 IC50 = 23.13 nM 7.64
CHEMBL5559387 IC50 = 43.44 nM 7.36
CHEMBL5537094 IC50 = 72.26 nM 7.14
CHEMBL5558221 IC50 = 74.51 nM 7.13
CHEMBL5517774 IC50 > 100.0 nM -
CHEMBL5549893 IC50 > 100.0 nM -