Assay Detail
Binding
CHEMBL5500853
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of HPK1 in human PBMC cells assessed as inhibition of SLP76 phosphorylation pretreated for 1 hr followed by anti-CD3/CD28 addition and measured after 1 hr by Western blot analysis
2
Total Activities
1
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | PBMC |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Mitogen-activated protein kinase kinase kinase kinase 1 (CHEMBL5749) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of 5-aminopyrido[2,3-d]pyrimidin-7(8H)-one derivatives as new hematopoietic progenitor kinase 1 (HPK1) inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 6.88 | 6.88 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5505932 | — | — | 2 | 6.88 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5505932 | — | IC50 | = | 131.8 | nM | 6.88 |
| CHEMBL5505932 | — | Inhibition | - | % | - |