Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5501445

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 10 mins by DTNB-reagent based Ellman's method
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Electrophorus electricus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL4078)
Type SINGLE PROTEIN
Organism Electrophorus electricus

Publication

Novel 6-alkyl-bridged 4-arylalkylpiperazin-1-yl derivatives of azepino[4,3-b]indol-1(2H)-one as potent BChE-selective inhibitors showing protective effects against neurodegenerative insults.
Samarelli F, Purgatorio R, Lopopolo G, Deruvo C, Catto M, Andresini M, Carrieri A, Nicolotti O, De Palma A, Miniero DV, de Candia M, Altomare CD.
Eur J Med Chem (2024) 270 : 116353 -116353
PubMed 38579622 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.72 7.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502 DONEPEZIL 4.0 1 7.68
CHEMBL95 TACRINE 4.0 1 6.54
CHEMBL5555131 1 6.40
CHEMBL5532253 1 5.83
CHEMBL5556474 1 5.74
CHEMBL5555332 1 5.31
CHEMBL5557312 1 5.23
CHEMBL5542336 1 5.18
CHEMBL5559422 1 5.15
CHEMBL5557601 1 5.04
CHEMBL5512467 1 4.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502 DONEPEZIL IC50 = 21.0 nM 7.68
CHEMBL95 TACRINE IC50 = 290.0 nM 6.54
CHEMBL5555131 IC50 = 402.0 nM 6.40
CHEMBL5532253 IC50 = 1480.0 nM 5.83
CHEMBL5556474 IC50 = 1830.0 nM 5.74
CHEMBL5555332 IC50 = 4940.0 nM 5.31
CHEMBL5557312 IC50 = 5900.0 nM 5.23
CHEMBL5542336 IC50 = 6570.0 nM 5.18
CHEMBL5559422 IC50 = 7060.0 nM 5.15
CHEMBL5557601 IC50 = 9100.0 nM 5.04
CHEMBL5512467 IC50 = 15000.0 nM 4.82