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Assay Detail

CHEMBL5506670

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal 6XHis-tagged/FLAG-tagged full length human recombinant HDAC1 (1 to 482 residues) expressed in baculovirus-infected Sf9 cells using Boc-Lys-(acetyl)-AMC as substrate preincubated with enzyme for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence analysis
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 1 (CHEMBL325)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

HDAC/NAMPT dual inhibitors overcome initial drug-resistance in p53-null leukemia cells.
Yue K, Sun S, Liu E, Liu J, Hou B, Qi K, Chou CJ, Jiang Y, Li X.
Eur J Med Chem (2024) 266 : 116127 -116127
PubMed 38224650 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.54 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5078985 1 8.52
CHEMBL5083249 1 8.45
CHEMBL5087431 1 8.42
CHEMBL5561596 1 8.26
CHEMBL5532146 1 8.12
CHEMBL5559648 1 8.09
CHEMBL5556752 1 8.00
CHEMBL5558823 1 7.90
CHEMBL5561465 1 7.88
CHEMBL5565699 1 7.75
CHEMBL5555272 1 7.75
CHEMBL5568033 1 7.60
CHEMBL5559070 1 7.60
CHEMBL98 VORINOSTAT 4.0 1 7.38
CHEMBL5557716 1 7.27
CHEMBL5560853 1 7.25
CHEMBL5532078 1 7.24
CHEMBL5550071 1 7.10
CHEMBL5563379 1 6.62
CHEMBL5557041 1 6.30
CHEMBL5532409 1 6.21
CHEMBL5560674 1 6.16
CHEMBL5560296 1 -
CHEMBL566757 DAPORINAD 2.0 1 -
CHEMBL5558311 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5078985 IC50 = 3.01 nM 8.52
CHEMBL5083249 IC50 = 3.52 nM 8.45
CHEMBL5087431 IC50 = 3.79 nM 8.42
CHEMBL5561596 IC50 = 5.5 nM 8.26
CHEMBL5532146 IC50 = 7.61 nM 8.12
CHEMBL5559648 IC50 = 8.17 nM 8.09
CHEMBL5556752 IC50 = 10.1 nM 8.00
CHEMBL5558823 IC50 = 12.7 nM 7.90
CHEMBL5561465 IC50 = 13.2 nM 7.88
CHEMBL5565699 IC50 = 18.0 nM 7.75
CHEMBL5555272 IC50 = 17.9 nM 7.75
CHEMBL5568033 IC50 = 25.1 nM 7.60
CHEMBL5559070 IC50 = 25.1 nM 7.60
CHEMBL98 VORINOSTAT IC50 = 41.9 nM 7.38
CHEMBL5557716 IC50 = 53.9 nM 7.27
CHEMBL5560853 IC50 = 56.1 nM 7.25
CHEMBL5532078 IC50 = 57.4 nM 7.24
CHEMBL5550071 IC50 = 79.3 nM 7.10
CHEMBL5563379 IC50 = 241.0 nM 6.62
CHEMBL5557041 IC50 = 500.0 nM 6.30
CHEMBL5532409 IC50 = 620.0 nM 6.21
CHEMBL5560674 IC50 = 689.0 nM 6.16
CHEMBL5560296 IC50 > 20000.0 nM -
CHEMBL566757 DAPORINAD IC50 > 10000.0 nM -
CHEMBL5558311 IC50 > 10000.0 nM -