Assay Detail
Binding
CHEMBL5507155
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of HPK1 in human Jurkat cells assessed as decrease in SLP-76 phosphorylation at Ser376 incubated for 1 hr by ELISA
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Jurkat |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Mitogen-activated protein kinase kinase kinase kinase 1 (CHEMBL5749) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of novel, potent, selective and orally bioavailable HPK1 inhibitor for enhancing the efficacy of anti-PD-L1 antibody.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 6.22 | 6.37 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5558185 | — | — | 1 | 6.37 |
| CHEMBL5555788 | — | — | 1 | 6.30 |
| CHEMBL5555659 | — | — | 1 | 6.27 |
| CHEMBL5558967 | — | — | 1 | 6.14 |
| CHEMBL5555917 | — | — | 1 | 6.01 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5558185 | — | IC50 | = | 424.05 | nM | 6.37 |
| CHEMBL5555788 | — | IC50 | = | 501.75 | nM | 6.30 |
| CHEMBL5555659 | — | IC50 | = | 534.65 | nM | 6.27 |
| CHEMBL5558967 | — | IC50 | = | 725.45 | nM | 6.14 |
| CHEMBL5555917 | — | IC50 | = | 977.7 | nM | 6.01 |