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Assay Detail

CHEMBL5507218

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK2 (unknown origin) using GFP-STAT1 as substrate incubated for 1 hrs by TR-FRET assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and evaluation of C-5 substituted pyrrolopyridine derivatives as potent Janus Kinase 1 inhibitors with excellent selectivity.
Chen L, Tang Y, Lang JJ, Lin Y, Yu Z, Li X, Zheng X, Mi P, Lv Y, Lin YW.
Eur J Med Chem (2024) 267 : 116210 -116210
PubMed 38359535 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.24 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5557208 1 9.00
CHEMBL5562462 1 8.66
CHEMBL5564520 1 8.18
CHEMBL5532094 1 7.78
CHEMBL5556990 1 7.65
CHEMBL221959 TOFACITINIB 4.0 1 7.42
CHEMBL3137308 PEFICITINIB 4.0 1 7.25
CHEMBL5558857 1 6.91
CHEMBL5556716 1 6.86
CHEMBL5542947 1 6.79
CHEMBL5532584 1 6.28
CHEMBL5542400 1 6.25
CHEMBL5566342 1 6.21
CHEMBL5563563 1 6.07
CHEMBL5560549 1 -
CHEMBL5561392 1 -
CHEMBL5564970 1 -
CHEMBL5563202 1 -
CHEMBL5559604 1 -
CHEMBL5561136 1 -
CHEMBL5561548 1 -
CHEMBL5568368 1 -
CHEMBL5559823 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5557208 IC50 = 1.0 nM 9.00
CHEMBL5562462 IC50 = 2.2 nM 8.66
CHEMBL5564520 IC50 = 6.6 nM 8.18
CHEMBL5532094 IC50 = 16.8 nM 7.78
CHEMBL5556990 IC50 = 22.4 nM 7.65
CHEMBL221959 TOFACITINIB IC50 = 38.3 nM 7.42
CHEMBL3137308 PEFICITINIB IC50 = 56.4 nM 7.25
CHEMBL5558857 IC50 = 123.0 nM 6.91
CHEMBL5556716 IC50 = 137.0 nM 6.86
CHEMBL5542947 IC50 = 162.0 nM 6.79
CHEMBL5532584 IC50 = 529.0 nM 6.28
CHEMBL5542400 IC50 = 556.0 nM 6.25
CHEMBL5566342 IC50 = 618.0 nM 6.21
CHEMBL5563563 IC50 = 845.0 nM 6.07
CHEMBL5560549 IC50 > 10000.0 nM -
CHEMBL5561392 IC50 > 10000.0 nM -
CHEMBL5564970 IC50 > 10000.0 nM -
CHEMBL5563202 IC50 > 10000.0 nM -
CHEMBL5559604 IC50 > 10000.0 nM -
CHEMBL5561136 IC50 > 10000.0 nM -
CHEMBL5561548 IC50 > 10000.0 nM -
CHEMBL5568368 IC50 > 10000.0 nM -
CHEMBL5559823 IC50 > 10000.0 nM -