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Assay Detail

CHEMBL5508745

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of USP2 (267 to 599 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using ubiquitin-rhodamine-110 as substrate preincubated with compound for 30 mins followed by substrate addition measured after 30 mins by fluorometric assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ubiquitin carboxyl-terminal hydrolase 2 (CHEMBL1293227)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of potent USP2/USP8 dual-target inhibitors for the treatment of breast cancer via structure guided optimization of ML364.
Tian Y, Liu K, Wu D, Wu L, Xu Q, Wei W, Li Z, Du Q, Bian J.
Eur J Med Chem (2024) 268 : 116275 -116275
PubMed 38452725 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.35 6.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5561761 1 6.10
CHEMBL5523555 1 6.05
CHEMBL5523445 1 5.89
CHEMBL5560776 1 5.85
CHEMBL5562321 1 5.82
CHEMBL5542690 1 5.80
CHEMBL5562765 1 5.50
CHEMBL5532378 1 5.48
CHEMBL5560773 1 5.47
CHEMBL3392741 1 5.44
CHEMBL5557100 1 5.42
CHEMBL5557814 1 5.36
CHEMBL5562196 1 5.34
CHEMBL5566850 1 5.26
CHEMBL5559526 1 5.26
CHEMBL5565871 1 5.23
CHEMBL5559268 1 5.19
CHEMBL5562787 1 4.98
CHEMBL5523454 1 4.88
CHEMBL5560170 1 4.84
CHEMBL5558678 1 4.81
CHEMBL5523517 1 4.65
CHEMBL5568379 1 4.54
CHEMBL5562286 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5561761 IC50 = 800.0 nM 6.10
CHEMBL5523555 IC50 = 890.0 nM 6.05
CHEMBL5523445 IC50 = 1300.0 nM 5.89
CHEMBL5560776 IC50 = 1400.0 nM 5.85
CHEMBL5562321 IC50 = 1500.0 nM 5.82
CHEMBL5542690 IC50 = 1600.0 nM 5.80
CHEMBL5562765 IC50 = 3200.0 nM 5.50
CHEMBL5532378 IC50 = 3300.0 nM 5.48
CHEMBL5560773 IC50 = 3400.0 nM 5.47
CHEMBL3392741 IC50 = 3600.0 nM 5.44
CHEMBL5557100 IC50 = 3800.0 nM 5.42
CHEMBL5557814 IC50 = 4400.0 nM 5.36
CHEMBL5562196 IC50 = 4600.0 nM 5.34
CHEMBL5566850 IC50 = 5500.0 nM 5.26
CHEMBL5559526 IC50 = 5500.0 nM 5.26
CHEMBL5565871 IC50 = 5900.0 nM 5.23
CHEMBL5559268 IC50 = 6400.0 nM 5.19
CHEMBL5562787 IC50 = 10500.0 nM 4.98
CHEMBL5523454 IC50 = 13300.0 nM 4.88
CHEMBL5560170 IC50 = 14500.0 nM 4.84
CHEMBL5558678 IC50 = 15400.0 nM 4.81
CHEMBL5523517 IC50 = 22300.0 nM 4.65
CHEMBL5568379 IC50 = 29100.0 nM 4.54
CHEMBL5562286 IC50 > 100000.0 nM -