Assay Detail
Binding
CHEMBL5508796
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of USP8 (unknown origin) using Di-Ub IQF K48-02 as substrate preincubated for 30 mins followed by substrate addition by high-throughput assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Ubiquitin carboxyl-terminal hydrolase 8 (CHEMBL2157854) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
The discovery of potent USP2/USP8 dual-target inhibitors for the treatment of breast cancer via structure guided optimization of ML364.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.02 | 6.02 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3392741 | — | — | 1 | 6.02 |
| CHEMBL5555634 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3392741 | — | IC50 | = | 950.0 | nM | 6.02 |
| CHEMBL5555634 | — | IC50 | < | 100.0 | nM | - |