Assay Detail
Binding
CHEMBL5509526
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity at N-terminal his6-tagged human recombinant NUDT5 (1 to 208 residues) expressed in Escherichia coli Rosetta (DE3) using ADP as substrate incubated for 1 hr by luminescence based microplate reader analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Unexpected Noncovalent Off-Target Activity of Clinical BTK Inhibitors Leads to Discovery of a Dual NUDT5/14 Antagonist.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.58 | 6.57 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5527860 | — | — | 1 | 6.57 |
| CHEMBL5549952 | — | — | 1 | 6.31 |
| CHEMBL1873475 | IBRUTINIB | 4.0 | 1 | 6.08 |
| CHEMBL1242204 | — | — | 1 | 5.69 |
| CHEMBL4072833 | EVOBRUTINIB | 3.0 | 1 | 4.86 |
| CHEMBL5549782 | — | — | 1 | 4.86 |
| CHEMBL4449342 | — | — | 1 | 4.67 |
| CHEMBL5560485 | — | — | 1 | - |
| CHEMBL5561533 | — | — | 1 | - |
| CHEMBL5565823 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5527860 | — | IC50 | = | 270.0 | nM | 6.57 |
| CHEMBL5549952 | — | IC50 | = | 487.0 | nM | 6.31 |
| CHEMBL1873475 | IBRUTINIB | IC50 | = | 837.0 | nM | 6.08 |
| CHEMBL1242204 | — | IC50 | = | 2040.0 | nM | 5.69 |
| CHEMBL4072833 | EVOBRUTINIB | IC50 | = | 13900.0 | nM | 4.86 |
| CHEMBL5549782 | — | IC50 | = | 13800.0 | nM | 4.86 |
| CHEMBL4449342 | — | IC50 | = | 21200.0 | nM | 4.67 |
| CHEMBL5560485 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL5561533 | — | IC50 | - | — | - | |
| CHEMBL5565823 | — | IC50 | - | — | - |