Assay Detail
Binding
CHEMBL5509530
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity at N-terminal his6-tagged human recombinant NUDT14 (1 to 222 residues) expressed in Escherichia coli Rosetta (DE3) using ADP as substrate incubated for 1 hr by luminescence based microplate reader analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Uridine diphosphate glucose pyrophosphatase NUDT14 (CHEMBL4105943) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Unexpected Noncovalent Off-Target Activity of Clinical BTK Inhibitors Leads to Discovery of a Dual NUDT5/14 Antagonist.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 6.15 | 6.79 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5527860 | — | — | 1 | 6.79 |
| CHEMBL5549952 | — | — | 1 | 6.58 |
| CHEMBL1242204 | — | — | 1 | 6.29 |
| CHEMBL1873475 | IBRUTINIB | 4.0 | 1 | 6.00 |
| CHEMBL5549782 | — | — | 1 | 5.79 |
| CHEMBL5561533 | — | — | 1 | 5.43 |
| CHEMBL5560485 | — | — | 1 | - |
| CHEMBL5565823 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5527860 | — | IC50 | = | 162.0 | nM | 6.79 |
| CHEMBL5549952 | — | IC50 | = | 263.0 | nM | 6.58 |
| CHEMBL1242204 | — | IC50 | = | 519.0 | nM | 6.29 |
| CHEMBL1873475 | IBRUTINIB | IC50 | = | 990.0 | nM | 6.00 |
| CHEMBL5549782 | — | IC50 | = | 1640.0 | nM | 5.79 |
| CHEMBL5561533 | — | IC50 | = | 3720.0 | nM | 5.43 |
| CHEMBL5560485 | — | IC50 | - | — | - | |
| CHEMBL5565823 | — | IC50 | - | — | - |