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Assay Detail

CHEMBL5511985

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human KOR expressed in CHO-K1 cells assessed as decrease in cAMP level incubated for 30 mins in presence of forskolin by HitHunter cAMP analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kappa-type opioid receptor (CHEMBL237)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Searching for Synthetic Opioid Rescue Agents: Identification of a Potent Opioid Agonist with Reduced Respiratory Depression.
Vu LY, Luo D, Johnson K, Denehy ED, Songrady JC, Martin J, Trivedi R, Alsum AR, Shaykin JD, Chaudhary CL, Woloshin EJ, Kornberger L, Bhuiyan N, Parkin S, Jiang Q, Che T, Alilain W, Turner JR, Bardo MT, Prisinzano TE.
J Med Chem (2024) 67 : 9173 -9193
PubMed 38810170 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 7.39 9.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL290543 1 9.27
CHEMBL3303774 1 8.44
CHEMBL2364605 CEBRANOPADOL 3.0 1 6.78
CHEMBL70 MORPHINE 4.0 1 6.46
CHEMBL596 FENTANYL 4.0 1 6.01
CHEMBL5523416 1 -
CHEMBL5568344 1 -
CHEMBL4467777 1 -
CHEMBL5542870 1 -
CHEMBL5568330 1 -
CHEMBL340032 DADLE 1 -
CHEMBL396460 NOCICEPTIN 1 -
CHEMBL573214 NORBINALTORPHIMINE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL290543 EC50 = 0.54 nM 9.27
CHEMBL3303774 EC50 = 3.65 nM 8.44
CHEMBL2364605 CEBRANOPADOL EC50 = 166.02 nM 6.78
CHEMBL70 MORPHINE EC50 = 345.67 nM 6.46
CHEMBL596 FENTANYL EC50 = 987.35 nM 6.01
CHEMBL5523416 EC50 > 10000.0 nM -
CHEMBL5568344 EC50 > 10000.0 nM -
CHEMBL4467777 EC50 > 10000.0 nM -
CHEMBL5542870 EC50 > 10000.0 nM -
CHEMBL5568330 EC50 > 10000.0 nM -
CHEMBL340032 DADLE EC50 - -
CHEMBL396460 NOCICEPTIN EC50 - -
CHEMBL573214 NORBINALTORPHIMINE EC50 - -