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Assay Detail

CHEMBL5513836

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human H1 receptor expressed in PathHunter CHO-K1 HRH1 beta-arrestin cells preincubated for 30 mins followed by agonist addition at 6 times EC80 and measured after 90 to 180 mins by PathHunter assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type PathHunter CHO-K1 HRH1 beta-arrestin
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of the novel and potent histamine H1 receptor antagonists for treatment of allergic diseases.
Chu Z, Cen L, Xu Q, Lin G, Mo J, Shao L, Zhao Y, Li J, Ye W, Fang T, Ren W, Zhu Q, He G, Xu Y.
Eur J Med Chem (2024) 268 : 116197 -116197
PubMed 38368709 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.63 9.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1172 DESLORATADINE 4.0 1 9.19
CHEMBL5557294 1 8.71
CHEMBL5565071 1 7.91
CHEMBL5560560 1 7.74
CHEMBL5556597 1 7.72
CHEMBL5568499 1 7.66
CHEMBL5559786 1 7.62
CHEMBL5542993 1 7.47
CHEMBL5567709 1 7.36
CHEMBL5555844 1 7.24
CHEMBL5558681 1 7.19
CHEMBL5554991 1 7.17
CHEMBL5532329 1 6.90
CHEMBL5555858 1 6.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1172 DESLORATADINE IC50 = 0.644 nM 9.19
CHEMBL5557294 IC50 = 1.97 nM 8.71
CHEMBL5565071 IC50 = 12.21 nM 7.91
CHEMBL5560560 IC50 = 18.36 nM 7.74
CHEMBL5556597 IC50 = 19.25 nM 7.72
CHEMBL5568499 IC50 = 21.81 nM 7.66
CHEMBL5559786 IC50 = 24.12 nM 7.62
CHEMBL5542993 IC50 = 33.91 nM 7.47
CHEMBL5567709 IC50 = 44.18 nM 7.36
CHEMBL5555844 IC50 = 57.23 nM 7.24
CHEMBL5558681 IC50 = 64.51 nM 7.19
CHEMBL5554991 IC50 = 66.93 nM 7.17
CHEMBL5532329 IC50 = 125.7 nM 6.90
CHEMBL5555858 IC50 = 125.7 nM 6.90