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Assay Detail

CHEMBL5516166

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IRE1alpha RNase activity (unknown origin) using XBP1 as substrate preincubated for 1 hr followed by thapsigargin stimulation for 5 hrs by One-Glo luciferase reagent based luminescence assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of Potent, Selective, and Orally Available IRE1α Inhibitors Demonstrating Comparable PD Modulation to IRE1 Knockdown in a Multiple Myeloma Model.
Braun MG, Ashkenazi A, Beveridge RE, Castanedo G, Wallweber HA, Beresini MH, Clark KR, De Bruyn T, Fu L, Gibbons P, Jiang F, Kaufman S, Kan D, Kiefer JR, Leclerc JP, Lemire A, Ly C, Segal E, Sims J, Wang W, Wei W, Zhao L, Schwarz JB, Rudolph J.
J Med Chem (2024) 67 : 8708 -8729
PubMed 38748820 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 6.51 7.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5555999 1 7.75
CHEMBL5562014 1 7.66
CHEMBL5557276 1 7.51
CHEMBL5557870 1 7.50
CHEMBL5565861 1 7.50
CHEMBL5558833 1 7.46
CHEMBL5563969 1 7.43
CHEMBL3356009 1 7.40
CHEMBL5542252 1 7.38
CHEMBL5561734 1 7.36
CHEMBL5561325 1 7.33
CHEMBL3356007 1 7.24
CHEMBL5560090 1 7.08
CHEMBL5555815 1 7.06
CHEMBL5562143 1 6.85
CHEMBL5532629 1 6.75
CHEMBL5542651 1 6.70
CHEMBL5555850 1 6.70
CHEMBL5557507 1 6.52
CHEMBL5556840 1 6.51
CHEMBL5523599 1 6.48
CHEMBL5532412 1 6.27
CHEMBL5555363 1 5.89
CHEMBL5560655 1 5.64
CHEMBL5565839 1 5.64
CHEMBL5567735 1 5.57
CHEMBL5560044 1 5.36
CHEMBL5560936 1 5.23
CHEMBL5523348 1 5.05
CHEMBL5561873 1 4.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5555999 IC50 = 18.0 nM 7.75
CHEMBL5562014 IC50 = 22.0 nM 7.66
CHEMBL5557276 IC50 = 31.0 nM 7.51
CHEMBL5557870 IC50 = 32.0 nM 7.50
CHEMBL5565861 IC50 = 32.0 nM 7.50
CHEMBL5558833 IC50 = 35.0 nM 7.46
CHEMBL5563969 IC50 = 37.0 nM 7.43
CHEMBL3356009 IC50 = 40.0 nM 7.40
CHEMBL5542252 IC50 = 42.0 nM 7.38
CHEMBL5561734 IC50 = 44.0 nM 7.36
CHEMBL5561325 IC50 = 47.0 nM 7.33
CHEMBL3356007 IC50 = 57.0 nM 7.24
CHEMBL5560090 IC50 = 84.0 nM 7.08
CHEMBL5555815 IC50 = 88.0 nM 7.06
CHEMBL5562143 IC50 = 140.0 nM 6.85
CHEMBL5532629 IC50 = 180.0 nM 6.75
CHEMBL5542651 IC50 = 200.0 nM 6.70
CHEMBL5555850 IC50 = 200.0 nM 6.70
CHEMBL5557507 IC50 = 300.0 nM 6.52
CHEMBL5556840 IC50 = 310.0 nM 6.51
CHEMBL5523599 IC50 = 330.0 nM 6.48
CHEMBL5532412 IC50 = 540.0 nM 6.27
CHEMBL5555363 IC50 = 1300.0 nM 5.89
CHEMBL5560655 IC50 = 2300.0 nM 5.64
CHEMBL5565839 IC50 = 2300.0 nM 5.64
CHEMBL5567735 IC50 = 2700.0 nM 5.57
CHEMBL5560044 IC50 = 4400.0 nM 5.36
CHEMBL5560936 IC50 = 5900.0 nM 5.23
CHEMBL5523348 IC50 = 9000.0 nM 5.05
CHEMBL5561873 IC50 = 26000.0 nM 4.58
CHEMBL5558080 IC50 = 28000.0 nM 4.55
CHEMBL5565965 IC50 = 32000.0 nM 4.50
CHEMBL5557082 IC50 > 50000.0 nM -