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Assay Detail

CHEMBL5516208

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against SARS-CoV-2 JPN/TY/WK-521 infected in VeroE6/TMPRSS2 cells assessed as reduction in viral RNA incubated with virus for 1 hr followed by compound addition and measured after 24 hrs by RT-PCR analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Severe acute respiratory syndrome coronavirus 2
Cell Type VeroE6/TMPRSS2
Confidence 1 — Organism
Curated By Autocuration

Target

SARS-CoV-2 (CHEMBL4303835)
Type ORGANISM
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Macrocyclic Azapeptide Nitriles: Structure-Based Discovery of Potent SARS-CoV-2 Main Protease Inhibitors as Antiviral Drugs.
Breidenbach J, Voget R, Si Y, Hingst A, Claff T, Sylvester K, Wolf V, Krasniqi V, Useini A, Sträter N, Ogura Y, Kawaguchi A, Müller CE, Gütschow M.
J Med Chem (2024) 67 : 8757 -8790
PubMed 38753594 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 5.67 6.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4802135 NIRMATRELVIR 4.0 1 6.07
CHEMBL5568269 1 5.68
CHEMBL5568382 1 5.67
CHEMBL5565472 1 5.59
CHEMBL5559068 1 5.59
CHEMBL5561728 1 5.41
CHEMBL5558707 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4802135 NIRMATRELVIR EC50 = 860.0 nM 6.07
CHEMBL5568269 EC50 = 2100.0 nM 5.68
CHEMBL5568382 EC50 = 2140.0 nM 5.67
CHEMBL5565472 EC50 = 2560.0 nM 5.59
CHEMBL5559068 EC50 = 2550.0 nM 5.59
CHEMBL5561728 EC50 = 3880.0 nM 5.41
CHEMBL5558707 EC50 - -